Not known Details About attune flow cytometer

Data-enriched characterization of cell populations. Morphological info from photos can increase to your richness of flow cytometry knowledge. For example, the determine reveals an otherwise regular apoptosis assay working with Annexin V and PI, including cell imaging to characterize cells in Each and every populace to expose morphologically unique attributes. These insights could not have been gained from multiplex staining by itself.

Whether you’re a newbie or an expert, take a look at our assets to advance your investigate and know-how in flow cytometry.

(B) RAW 264.7 macrophages are frequently annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The number of singlets obtainable for Assessment improves once the person trains and employs a more exact design.

The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing which has a substantial-velocity brightfield digital camera for simultaneous higher throughput flow cytometry and superior resolution brightfield imaging.

*Pricing is indicative only and is particularly subject to vary. Remember to Get hold of your Agilent agent with any queries. The data set forth herein will not be a proposal or even a dedication, equally of that are issue to credit acceptance, stipulations, and availability with Agilent. Agilent Economic Options is obtainable in chosen international locations.

Right here, a skilled person has gated singlets confidently. Immediately after evaluating the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of in excess of four% aggregates.

Pair your Attune flow cytometer with an autosampler for increased workflow efficiency, and insert robotic integration for max throughput.

Dependable impression high quality even at higher throughput Acoustic concentrating and a substantial-velocity camera Mix to impression these T cells constantly at minimal or superior flow rates. Effortlessly alter focus and camera options to satisfy experimental needs.

The Attune NxT cytometer is the dependable workhorse instrument your lab can rely upon for dependable and trusted overall performance. The Attune CytPix model includes a substantial-speed brightfield camera and AI-pushed graphic Examination software program, with new updates such as equipment Mastering applications and an intuitive person interface, enabling enhanced data precision, accuracy, and faster insights into sample biology.

End users can view celebration photos in actual-time to confirm sample quality, improve protocols, and document the morphology of each cell population discovered for Evaluation. Imaging seize also benefits from acoustic concentrating, which helps you to posture the cells so that a sharp, centered image is obtained.

Audio waves help exact cell alignment, causing Fantastic info high-quality and ~10X more rapidly sample flow costs.

We Beforehand shown the power of imaging Vehicle-T/Ramos mobile interactions. Enable’s have a look at just the populace of best fascination, the double positive activities, To find out more. We can easily now use prolonged morphological parameters (right here, circularity and skewness of depth) to additional study the characteristics of these populations and refine gating on these occasions, escalating facts robustness.

Designed-in dimensionality reduction applications increased resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to substantiate anticipated inhabitants resolution in lysed whole human blood. Person can perspective activities from Every single cluster for visual affirmation of mobile phenotypes.

Adaptable payment prepare from Agilent Monetary Remedies permit you to have the vital instrumentation you will need for quick click here task, or to develop your lab.

Gating on these prolonged parameters helps you to swiftly and properly discover populations of desire and confirm gating tactic with little if any manual evaluation. The Attune software program provides entry to condition-of-the-artwork device Discovering analysis applications in just an expertly built person interface, rendering it extremely simple to operate.

Since the cell line experienced previously been analyzed on the Attune CytPix Flow Cytometer, the scientists went again to the photographs and have been ready to doc the microbial infection at the very least 5 days previously.

The Attune CytPix Flow Cytometer allows people to visualise samples during acquisition, verify the accuracy of gating methods, and assistance benefits with responsible documentation for publication functions.

Below, we exhibit that by gating methods working with graphic-centered quantitative parameters can distinguish interacting cells from coincident activities to more accurately examine interacting cells.